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PhD position in UK

Deciphering host-oral microbiome interactions and mechanisms for prevention of dysbiosis and antimicrobial resistance

Type of research degree: PhD
Application deadline: Thursday 29 April 2021

Summary
Host-microbiome interactions play a key role in determining health and disease status. By deciphering the intricate signals within host and microbiome profiles we will advance knowledge for maintaining health and preventing dysbiosis. The significance of the microbiome on host health has led to the emergence of new therapeutic approaches focused on the prescribed manipulation of the host microbiome, either by decreasing harmful taxa or reinstating missing beneficial taxa and the functional roles they perform.

Full description
A rich and diverse microbiome is necessary for host organisms, as it contributes to the smooth development and functioning of important physiological processes. We will focus on the oral microbiota and its role maintaining the oral mucosal barrier. Reducing the portals for systemic translocation of organisms and disruption to systemic health from ensuing infections. This is particularly problematic in individuals whose immune system is compromised, highlighting the importance of understanding the underlying biological mechanisms at work.

The oral cavity comprises microbes that attach to surfaces in communities called biofilms. These are highly regulated with complex interdependency of organisms that adapt to changes in the wider environment2. These biofilms are essential to health with multiple functions e.g. contributing to the maintenance of mucosa barrier that prevents invasion of disease-promoting species. Co-cultures allow for the study of cell–cell interactions that more closely mimic complex tissue structures (e.g. human cells–biofilm). Numerous studies are advancing understanding of the role of the microbiome as a therapeutic agent and its significance in human health.

We hypothesise that the oral microbiome signatures and associated host responses are distinct in health and in dysbiosis. Microbiome modulation strategies can prevent dysbiotic events and maintain health.

Aims and Objectives
To advance current understanding of the taxonomic and functional profiles of the oral microbiome in health and in dysbiosis, and screen for antimicrobial resistance genes harboured by the oral microbiome.
To construct and characterise co-cultured biofilms with oral epithelial cells.
To design in silico pipelines for oral health and prevention of dysbiosis modelling, using a deep learning approach.
This BBSRC-funded iCASE studentship is a collaboration between Dr Do and Prof Pavitt at the University of Leeds, and GlaxoSmithKline Consumer Healthcare (GSK).

You will be trained in human cell and oral biofilm co-culture, molecular methods of metagenomic and transcriptomic profiling, and bioinformatics, using state-of-the art facilities at the University of Leeds. Throughout the project, you will collaborate closely with highly experienced research scientists at both Leeds and GSK. As a BBRSC iCASE student, you will be expected to spend three months on secondment at GSK Oral Health Research and Development (R&D) facility in Weybridge.

The University of Leeds is committed to recruiting exceptional postgraduate researchers regardless of age, ethnicity, gender, gender identity, disability, sexual orientation or career pathway to date. Our recruitment process is built to ensure equal opportunity, and we thrive to provide inclusive research-led curriculum, to enable all our students to achieve their future study and career aspirations. We welcome applicants from all backgrounds, particularly those underrepresented in science, who have curiosity, creativity and a drive to learn new skills.

References
Do T. 2017. Insights into microbial ecosystems using a new computational approach. Oral Diseases. 23(7), pp. 817-819.
Kilian M, Chapple ILC, Hannig M, et al. The oral microbiome – an update for oral healthcare professionals. BDJ. 2016;221:657–666.
Cheng Z, Do T, Mankia K, Meade JL, Hunt L, Clerehugh V, Speirs A, Tugnait A, Emery P, Devine DA. Dysbiosis in the Oral Microbiomes of anti-CCP Positive Individuals at Risk of Developing Rheumatoid Arthritis. 2021 Feb;80(2):162-168. doi: 10.1136/annrheumdis-2020-216972.

How to apply
To apply for this scholarship applicants should complete a Faculty Scholarhip Application Form and send this alongside a full academic CV, degree certificates and transcripts (or marks so far if still studying) to the Faculty Graduate School [email protected]

We also require 2 academic references to support your application. Please ask your referees to send these references on your behalf, directly to [email protected] by no later than Thursday 29 April 2021

If English is not your first language, you must provide evidence that you meet the University’s minimum English language requirements (below).

We welcome applications from all suitably-qualified candidates, but UK black and minority ethnic (BME) researchers are currently under-represented in our Postgraduate Research community, and we would therefore particularly encourage applications from UK BME candidates. All scholarships will be awarded on the basis of merit.

Entry requirements
You should hold a first degree equivalent to at least a UK upper second class honours degree in a relevant subject. This project is ideally suited to a candidate who wants to work at the interface of Academia and Industry. Background in molecular microbiology, and knowledge of computer programming (such as Linux bash scripting, Python and R) are desirable.

English language requirements
Applicants whose first language is not English must provide evidence that their English language is sufficient to meet the specific demands of their study. The Faculty of Medicine and Health minimum requirements in IELTS and TOEFL tests for PhD, MSc, MPhil, MD are: • British Council IELTS – score of 6.5 overall, with no element less than 6.0 • TOEFL iBT – overall score of 92 with the listening and reading element no less than 21, writing element no less than 22 and the speaking element no less than 23.

Funding on offer
The BBSRC iCASE PhD studentship is available for applicants who are eligible to pay academic fees at the UK fee rate. The studentship will attract an annual tax-free stipend of £15,285 for up to 4 years, subject to satisfactory progress and will cover the academic fee at the UK rate.

Contact details
For further information please contact the Faculty Graduate School
e: [email protected]

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